Exploitation and dissimination activities of the SAFOTEST The following publications

Exploitation and dissimination activities of the SAFOTEST

The following publications, posters and presentations have been held, prepared or published during the first, second and third reporting period of the SAFOTEST-project:

Publications
Baumgartner P, Raemaekers RJM, Durieux A, Gatehouse A, Davies H and Taylor M. (2002) Large-scale production, purification and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Protein Expression and Purification 26, 394-405.

Frenzel, Th., Miller, A., Engel, K.-H.
Neue Methoden zur analytischen Charakterisierung gentechnisch veränderter Lebensmittel - "Profiling" von Reisinhaltsstoffen.
Lebensmittelchemie 55, 96 (2001)

Frenzel, Th., Miller, A., Engel, K.-H.
Metabolite profiling: Analysis of major and minor rice grain constituents by means of GC-FID/MS and automatic data treatment.
In: Pfannhauser, W., Fenwick, G.R., and Khokhar, S. (eds.), Biologically-active phytochemicals in food. The Royal Society of Chemistry, Cambridge, UK, p. 271 - 273 (2001)

Frenzel, Th., Miller, A., Engel, K.-H.
Sicherheitsbewertung gentechnisch veränderter Lebensmittel: Eine "Metabolite Profiling"-Methode zur Erfassung von Haupt- und Minorbestandteilen in Reis.
Lebensmittelchemie 56, 17 (2002)

Frenzel, Th., Miller, A., Engel, K.-H.
Metabolite profiling - a fractionation method for analysis of major and minor compounds in rice grains.
Cereal Chem. 79(2), 215-221 (2002)
Frenzel, Th., Miller, A., Engel, K.-H.
A methodology for automated comparative analysis of metabolite profiling data.
Eur. Food Res. Technol., DOI 10.1007/s00217-002-0659-y

Kok, E., Peijnenburg, A., Keijer, J., and Kuiper, H. (2002) Toepassing van de microarraytechnologie bij de veiligheidsbeoordeling van genetisch gemodificeerde voedingsmiddelen. Voeding Nu 11, 21-23.

Kleter, G.A., and Peijnenburg, A.A.C.M. (2002) Screening of transgenic proteins expressed in transgenic food crops for the presence of short amino acid sequences identical to potential, IgE – binding linear epitopes of allergens. BMC Structural Biology 2: 8

Miller, A., Frenzel, Th., Schmarr, H.-G., Engel, K.-H.
Analytik von g-Oryzanol in Reis mittels on-line LC-GC.
Lebensmittelchemie 56, 92-93 (2002)

Miller, A., Frenzel, Th., Engel, K.-H.
g-Oryzanol - eine Modellstudie zur analytischen Charakterisierung gentechnisch veränderter Lebensmittel.
Lebensmittelchemie 57, 11 (2003)

Miller, A., Frenzel, Th., Schmarr, H.-G., Engel, K.-H.
Coupled liquid chromatography-gas chromatography for the rapid analysis of g-oryzanol in rice lipids.
J. Chromatogr. A 985, 403-410 (2003)

Miller, A., Frenzel, Th., Schmarr, H.-G., Engel, K.-H.
Development of an on-line LC-GC method for the rapid analysis of g-oryzanol in rice bran oil.
In: Pfannhauser, W., Fenwick, G.R., and Khokhar, S. (eds.), Biologically-active phytochemicals in food. The Royal Society of Chemistry, Cambridge, UK, p. 274 - 276 (2001)

Noteborn, H.P.J.M., Peijnenburg, A. (2000) Functional genomics: a platform for the risk-benefit assessment of food chemicals. In: "Report Novel Approaches to Risk-Benefit analysis in Food Chemical Safety", BBSRC-FSA Workshop, Norwich 4-5 July, 2000.

Nur, T., Peijnenburg, A., Noteborn, H.P.J.M., Baykus, H., and Reifen, R. (2002) Similar gene expression patterns in vitamin A deficiency and chemically-induced colitis in rats as revealed by DNA microarray technology. J. Nutr. 132, 2131-2136.

Peijnenburg, A., Noteborn, H.P.J.M., and Kuiper, H.A. (2002) Biomarkers for the safety evaluation of genetically modified foods. In "Biomarkers of Disease: An Evidence-Based Approach" (Trull, A. et al., eds.), pp. 313-321. Cambridge University Press.

Posters
Miller, A., Frenzel, Th., Schmarr, H.-G., Engel, K.-H.
Development of an on-line LC-GC method for the rapid analysis of g-oryzanol in rice bran oil.
EUROFOODCHEM XI meeting, 26 - 28 September 2001, Norwich, UK

Frenzel, Th., Miller, A., Engel, K.-H.
Metabolite profiling: analysis of major and minor rice grain constituents by means of GC-FID/MS and automatic data treatment.
EUROFOODCHEM XI meeting, 26 - 28 September 2001, Norwich, UK

Frenzel, Th., Miller, A., Engel, K.-H.
Metabolite profiling: Analysis of major and minor rice grain constituents by means of GC-FID/MS and automated data treatment.
Deutscher Lebensmittelchemikertag, 10 - 12 September 2001, Braunschweig, Germany

Peijnenburg, A., Baykus H., Steensma A. and Noteborn, H.
Analysis of gene expression in Caco-2 cells using cDNA microarrays.
Centre for Human Nutrigenomics, 5. September 2001, Wageningen, Holland.

Frenzel, Th., Miller, A., Altosaar, I., Shu, Q., Engel, K.-H.
New methods for the safety testing of GM foods: metabolite profiling of Bt-rice.
First International Congress on Plant Metabolomics, 7 - 11 April 2002, Wageningen, Holland

Miller, A., Frenzel, Th., Engel, K.-H.
g-Oryzanol in Reis - eine Modellstudie zur analytischen Charakterisierung konventioneller und neuartiger Lebensmittel.
31. Deutscher Lebensmittelchemikertag, 9 - 11 September 2002, Frankfurt/M., Germany

Diploma and Bachelor Thesis
Beate Zuzan
Entwicklung einer NIR spektroskopischen Methode zur Bestimmung von Phytinsäure in Braunreis
Diploma Thesis, Technical University Munich, 2001
Elke Müller
Beiträge zur Validierung einer Metabolite Profiling Methode für Reis
Bachelor Thesis, Technical University Munich, 2001
Richard Röhlig
Beiträge zur Entwicklung einer Metabolite Profiling Methode für Reis
Semester Thesis, Technical University Munich, 2002
Anke Scheffler
Metabolite Profiling von Gersteninhaltsstoffen im Verlauf des Mälzungsprozesses
Semester Thesis, Technical University Munich, 2002
Michael Kreiter
Beiträge zur Entwicklung einer NIRS-Methode zur Bestimmung von Phytinsäure in Braunreis
Semester Thesis, Technical University Munich, 2002

Internet
Tools for automated comparative analysis of metabolite profiling data were developed in the course of SAFOTEST. Software is available at http://www.wzw.tum.de/blm/alt/metabolite_profiling/index.php